Article

An efficient lentiviral CRISPRi approach to silence genes in primary human monocytes

2020-12-24

Abstract excerpt

Human primary monocytes are critical in controlling immune responses and tissue homeostasis. However, to identify and study molecular components that underlie the function of these cells, there remains a need to develop methods to perturb genes in these cells. Here we report lentiviral-based delivery of dCas9-KRAB and guide RNAs to efficiently silent target genes, such as CD45 and CD209 . We further show that sg...

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Literature Corpus work: 484698f2-6f0d-59c3-9989-b419bd2e8490
DOI: 10.1101/2020.12.23.424242

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