5′ Modifications Improve Potency and Efficacy of DNA Donors for Precision Genome Editing

Abstract excerpt

Nuclease-directed genome editing is a powerful tool for investigating physiology and has great promise as a therapeutic approach to correct mutations that cause disease. In its most precise form, genome editing can use cellular homology-directed repair (HDR) pathways to insert information from an exogenously supplied DNA repair template (donor) directly into a targeted genomic location. Unfortunately, particularly...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• DNA Breaks, Double-Stranded
• Gene Editing
• Germ Cells
• Mutation
• Precision Medicine

Identifiers and source

Literature Corpus work

4472f0eb-0498-5c87-9822-72fc1a43473a

DOI

10.1101/354480