Article

Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity

2022-07-06

Abstract excerpt

CRISPR/Cas9 and CRISPR/Cas12a are characterized by a single-component effector protein. Despite unique features like DNA cleavage with 5′ staggered ends and a single crRNA, Cas12a has not been adopted in therapeutics to the same extent as Cas9. To address the pressing therapeutic needs for effective CRISPR/Cas12 systems, we selected two candidates, designated mgCas12a-1 and mgCas12a-2, from an analysis of the huma...

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Identifiers and source

Literature Corpus work: 40108715-c96c-5aea-a174-14afe0d893a5
DOI: 10.21203/rs.3.rs-1720912/v1

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