Article

Salt-dependent non-catalytic allostery of human USP14-regulated 26S proteasome

2024-11-07

Abstract excerpt

USP14, a deubiquitylating enzyme, regulates 26S proteasome function both catalytically and non-catalytically through multiple checkpoints. How USP14 non-catalytically regulates the proteasome activity remains elusive. Here, we combined genetic engineering and cryo-EM to disentangle how USP14 non-catalytically regulates proteasome activity in a salt-dependent manner. By solving 16 high-resolution cryo-EM structures...

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Literature Corpus work: 3f6802d9-732e-570c-bbbf-7d3ca7907b89
DOI: 10.1101/2024.11.07.622408

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