An in vitro workflow to create and modify novel infectious clones using replication cycle reaction

Abstract excerpt

<title>Abstract</title> <p>Reverse genetics systems are a critical tool in combating emerging viruses by enabling a better understanding of the genetic mechanisms by which they cause disease. Traditional cloning approaches are fraught with difficulties due to the bacterial toxicity of many viral sequences. Many attempts have been made to develop a simplified workflow to produce a simple-to-use infectious clone wi...

Topics

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• Animal Disease Management and Epidemiology
• COVID-19
• CRISPR and Genetic Engineering
• Mosquito-borne diseases and control
• Replicon
• RNA Interference and Gene Delivery
• SARS-CoV-2 and COVID-19 Research
• SARS-CoV-2 detection and testing

Identifiers and source

Literature Corpus work

3d27a462-524b-5d12-82ae-b9f1637e6340

DOI

10.21203/rs.3.rs-1894417/v1