Replication and single-cycle delivery of SARS-CoV-2 replicons.

doi:10.1126/science.abj8430

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Article

Replication and single-cycle delivery of SARS-CoV-2 replicons.

Science (New York, N.Y.) - 26 Nov 2021

Ricardo-Lax Inna, Luna Joseph M, Thao Tran Thi Nhu, Le Pen Jérémie, Yu Yingpu, Hoffmann H-Heinrich, Schneider William M, Razooky Brandon S, Fernandez-Martinez Javier, Schmidt Fabian, Weisblum Yiska, Trüeb Bettina Salome, Berenguer Veiga Inês, Schmied Kimberly, Ebert Nadine, Michailidis Eleftherios, Peace Avery, Sánchez-Rivera Francisco J, Lowe Scott W, Rout Michael P, Hatziioannou Theodora, Bieniasz Paul D, Poirier John T, MacDonald Margaret R, Thiel Volker, Rice Charles M

Abstract excerpt

Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These...

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Identifiers

PMID: 34648371
DOI: 10.1126/science.abj8430
PMCID: PMC9007107

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