Article

Development, applications, and future prospects of RNA virus reverse genetics technology

2026-07-30

Abstract excerpt

Viral reverse genetics enables the rescue of infectious virions from cloned cDNA and serves as a core technique for mapping viral genotype–phenotype relationships, dissecting RNA viral life cycles, and developing antiviral countermeasures. This review systematically summarizes the evolution, technical framework and optimization strategies of RNA virus reverse genetics, with three prototype viruses covering all maj...

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Literature Corpus work: f41d5b57-520e-5efa-ad34-150f0611c4da
DOI: 10.3389/fviro.2026.1897615

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