Article

CRISPR-based shuttle cloning of 1,397 human genes into UAS vectors

2025-02-16

Abstract excerpt

<h4>ABSTRACT</h4> The construction of genome-wide DNA libraries from publicly available resources is essential for leveraging functional genomics to investigate complex biological systems. However, all existing high-throughput cloning methods for transferring DNA fragments between vectors require PCR amplification of the DNA fragments, rendering the construction of genome-wide DNA libraries labor-intensive and ti...

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Literature Corpus work: 35eb0da1-0c40-50e3-9cc7-3363ab4c5d72
DOI: 10.1101/2025.02.16.638485

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