Article

Translation uniformity, evolutionary conservation, and peptide properties associate with microprotein mass spectrometry detection

2026-05-08

Abstract excerpt

<title>Abstract</title> <p> <bold>Background</bold> Non-canonical open reading frames (ncORFs) encode a substantial, largely unexplored layer of the human proteome. The short microproteins they produce frequently escape detection by standard mass spectrometry (MS) workflows, creating a “dark proteome” that hampers gene annotation and functional characterisation. To address this, sequence-derived attributes were...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 32359c8d-c008-5208-966b-3c54427499e8
DOI: 10.21203/rs.3.rs-9645389/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Translation uniformity, evolutionary conservation, and peptide properties associate with microprotein mass spectrometry detectionDOI 10.21203/rs.3.rs-9645389/v1Read

Detection of human unannotated microproteins by mass spectrometry-based proteomics: a community assessmentSemantic proximity Read

Thousands of novel unannotated proteins expand the MHC I immunopeptidome in cancerSemantic proximity Read

High-quality peptide evidence for annotating non-canonical open reading frames as human proteinsSemantic proximity Read

Sequence-intrinsic barriers define a class of translation-restricted uORFs in the human genomeSemantic proximity Read

What can Ribo-seq and proteomics tell us about the non-canonical proteome?Semantic proximity Read

Most non-canonical proteins uniquely populate the proteome or immunopeptidomeSemantic proximity Read

CrypticProteinDB: an integrated database of proteome and immunopeptidome derived non-canonical cancer proteinsSemantic proximity Read

An expanded reference catalog of translated open reading frames for biomedical researchSemantic proximity Read

Protein Language Models Rescue Variant Pathogenicity Prediction in Intrinsically Disordered Regions Through Synergistic Integration with Structure-Based MethodsSemantic proximity Read

ESMRank reveals a transferable axis of protein mutational constraint from overlapping variant effect assaysSemantic proximity Read

A deep audit of the PeptideAtlas database uncovers evidence for unannotated coding genes and aberrant translationSemantic proximity Read

Global proteogenomic analysis of human MHC class I-associated peptides derived from non-canonical reading frames.Semantic proximity Read