Article

High-throughput characterization of photocrosslinker-bearing ion channel variants to map residues critical for function and pharmacology

2020-11-24

Abstract excerpt

Incorporation of non-canonical amino acids (ncAAs) can endow proteins with novel functionalities, such as crosslinking or fluorescence. In ion channels, the function of these variants can be studied with great precision using standard electrophysiology, but this approach is typically labor intensive and low throughput. Here, we establish a high-throughput protocol to conduct functional and pharmacological investig...

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Literature Corpus work: 28cc4604-8217-58fb-a326-f2f58025e42f
DOI: 10.1101/2020.11.24.392498

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