Article
Gating transitions in the palm domain of ASIC1a.
The Journal of biological chemistry - 22 Feb 2013
Della Vecchia Margaret C, Rued Anna C, Carattino Marcelo D
Abstract excerpt
Acid-sensing ion channels (ASICs) are trimeric cation-selective proton-gated ion channels expressed in the central and peripheral nervous systems. The pore-forming transmembrane helices in these channels are linked by short loops to the palm domain in the extracellular region. Here, we explore the contribution to proton gating and desensitization of Glu-79 and Glu-416 in the palm domain of ASIC1a. Engineered Cys,...
Topics
- Acid Sensing Ion Channels
- Animals
- Cysteine
- Electrophysiology
- Epitopes
- Glutamic Acid
- Hydrogen-Ion Concentration
- Hydrolysis
- Ion Channel Gating
- Mice
- Molecular Conformation
- Mutation
- Protein Conformation
- Protein Structure, Tertiary
- Protons
- Xenopus