Back to search

Article

Adaptation to <i>ex vivo</i> culture drives human haematopoietic stem cell loss of repopulation capacity in a cell cycle independent manner

2022-11-17

Abstract excerpt

Loss of long-term haematopoietic stem cell function (LT-HSC) hampers the success of ex vivo HSC gene therapy and expansion procedures, but the kinetics and the mechanisms by which this occurs remain incompletely characterized. Here through time-resolved scRNA-Seq, matched in vivo functional analysis and the use of a reversible in vitro system of early G 1 arrest, we define the sequence of transcriptional and f...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
27fe97cc-2114-5a77-963e-701f1ba64b7b
DOI
10.1101/2022.11.17.516906
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Adaptation to <i>ex vivo</i> culture drives human haematopoietic stem cell loss of repopulation capacity in a cell cycle independent mannerDOI 10.1101/2022.11.17.516906
Adaptation to ex vivo culture reduces human hematopoietic stem cell activity independently of the cell cycleSemantic proximity
Human Haematopoietic Stem Cells released into circulation following mobilization express multiple G0-associated quiescence markersSemantic proximity
<i>Ex vivo</i> Expansion Potential of Murine Hematopoietic Stem Cells: A Rare Property Only Partially Predicted by PhenotypeSemantic proximity
<i>In situ</i> transcriptome characteristics are lost following culture adaptation of adult cardiac stem cellsSemantic proximity
Transcriptional landscape of direct reprogramming toward the hematopoietic lineageSemantic proximity
A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemnessSemantic proximity
Global genome decompaction leads to stochastic activation of gene expression as a first step toward fate commitment in human hematopoietic stem cellsSemantic proximity
NOTCH-mediated ex vivo expansion of human hematopoietic stem and progenitor cells by culture under hypoxiaSemantic proximity
In situ transcriptome characteristics are lost following culture adaptation of adult cardiac stem cellsSemantic proximity
Integrated time-lapse and single-cell transcription studies highlight the variable and dynamic nature of human hematopoietic cell fate commitmentSemantic proximity
Long-term lineage commitment in haematopoietic stem cell gene therapySemantic proximity
Divisional history and hematopoietic stem cell function during homeostasis.Semantic proximity
Select a neighboring publication to make it the new centre.