Article

Recovery of histamine H3 receptor activity lost in yeast cells through error-prone PCR and in vivo selection

2023-07-31

Abstract excerpt

G protein-coupled receptors (GPCRs) are the largest protein family in humans and are drug targets. Yeast, especially Saccharomyces cerevisiae , is a useful host for modifying the function and stability of GPCRs through protein engineering, which is advantageous over mammalian cells. When GPCRs are expressed in yeast, their function is often impaired. In this study, we performed random mutagenesis using error-prone...

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Literature Corpus work: 26900ad7-f087-5473-9a2a-45ef6c123a00
DOI: 10.21203/rs.3.rs-3193563/v1

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