Article

Bioluminescence-based reporters for characterizing inhibitors and activators of human Sonic Hedgehog protein autoprocessing in live cells at high throughput

2022-06-27

Abstract excerpt

<h4>ABSTRACT</h4> The Sonic hedgehog (SHh) precursor protein undergoes biosynthetic autoprocessing to cleave off and cholesterylate the SHh signaling ligand, a vital morphogen and oncogenic effector protein. Autoprocessing is self-catalyzed by SHhC, the SHh precursor’s enzymatic domain. Here we describe the development and validation of the first cellular reporter to monitor human SHhC autoprocessing non-invasive...

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Literature Corpus work: 238f4438-be30-5b76-bbe4-fb0b0571e205
DOI: 10.1101/2022.06.27.497760

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