Article

Single-molecule multimodal timing of <i>in vivo</i> mRNA synthesis

2025-04-29

Abstract excerpt

mRNA synthesis requires extensive pre-mRNA maturation, the organisation of which remains unclear. Here, we directly sequence pre-mRNA without metabolic labelling or amplification to resolve transcription and multimodal pre-mRNA processing at single-molecule resolution. Using poly(A) tail measurement, we distinguish transcriptionally engaged pre-mRNA from polyadenylated transcripts, revealing that splicing is subst...

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Literature Corpus work: 21ff1802-64b9-593d-8d52-3991b07e8dae
DOI: 10.1101/2025.04.27.650906

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