Article

A novel long-amplicon <i>rpoB</i> primer pair for high resolution microbiome analysis at the species-level

2026-05-17

Abstract excerpt

<h4>ABSTRACT</h4> The 16S rRNA gene is the most widely used genetic marker for microbial community profiling, but its limited sequence divergence often prevents species-level identification. The RNA polymerase β-subunit gene ( rpoB ) offers higher sequence variability, single-copy occurrence, and stronger phylogenetic consistency, yet its adoption in metataxonomic studies has been constrained by the lack of univ...

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Literature Corpus work: 210f7e81-87ad-5ff9-a36f-fd6c8984249e
DOI: 10.64898/2026.05.15.725465

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