Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies.

Applied and environmental microbiology - 1 Jan 2007

Case Rebecca J, Boucher Yan, Dahllöf Ingela, Holmström Carola, Doolittle W Ford, Kjelleberg Staffan

Abstract excerpt

Several characteristics of the 16S rRNA gene, such as its essential function, ubiquity, and evolutionary properties, have allowed it to become the most commonly used molecular marker in microbial ecology. However, one fact that has been overlooked is that multiple copies of this gene are often present in a given bacterium. These intragenomic copies can differ in sequence, leading to identification of multiple...

Topics

Bacteria
Base Sequence
DNA-Directed RNA Polymerases
Ecology
Ecosystem
Evolution, Molecular
Genes, rRNA
Genetic Markers
Genetic Variation
Molecular Sequence Data
Phylogeny

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Ribosomal, 16S

Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 17071787
DOI: 10.1128/AEM.01177-06
PMCID: PMC1797146

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-17071787-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies.PMID 17071787Read ↗

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity.Semantic proximity Read ↗

A novel long-amplicon <i>rpoB</i> primer pair for high resolution microbiome analysis at the species-levelSemantic proximity Read ↗

Microbial phylogeny and diversity: small subunit ribosomal RNA sequence analysis and beyond.Semantic proximity Read ↗

<i>rpoB</i> sequence analysis as a novel basis for bacterial identificationSemantic proximity Read ↗

Broad-range amplification and sequencing of the rpoB gene: a novel assay for bacterial identification in clinical microbiologySemantic proximity Read ↗

Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis.Semantic proximity Read ↗

Molecular approaches: advantages and artifacts in assessing bacterial diversity.Semantic proximity Read ↗

rpoB gene as a novel molecular marker to infer phylogeny in Planctomycetales.Semantic proximity Read ↗

Ecological significance of microdiversity: identical 16S rRNA gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies.Semantic proximity Read ↗

16SpeB: Towards defining bacterial species boundaries by intra-species gene sequence identitySemantic proximity Read ↗

Ribosomal DNA sequencing as a tool for identification of bacterial pathogensSemantic proximity Read ↗

Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.