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Broad-range amplification and sequencing of the rpoB gene: a novel assay for bacterial identification in clinical microbiology

17 Jun 2024

Abstract excerpt

ABSTRACT The rpoB gene has been proposed as a promising phylogenetic marker for bacterial identification, providing theoretically improved species-level resolution compared to the 16S rRNA gene for a range of clinically important taxa. However, its utility in diagnostic microbiology has been limited by the lack of broad-range primers allowing for its amplification from most species with a single PCR assay. Here,...

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PMID: 38884485
DOI: 10.1128/jcm.00266-24

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