Article
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies.
Nucleic acids research - 7 Jan 2013
Klindworth Anna, Pruesse Elmar, Schweer Timmy, Peplies Jörg, Quast Christian, Horn Matthias, Glöckner Frank Oliver
Abstract excerpt
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs were evaluated in silico with respect to the SILVA 16S/18S rDNA non-redundant reference dataset (SSURef 108 NR)....
Topics
- Archaea
- Bacteria
- Biodiversity
- Computer Simulation
- DNA Primers
- Genes, rRNA
- Genetic Variation
- High-Throughput Nucleotide Sequencing
- Metagenome
- Polymerase Chain Reaction