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KuafuPrimer: Machine learning empowers the design of 16S amplicon sequencing primers toward minimal bias for bacterial communities

2026-03-31

Abstract excerpt

Amplicon sequencing protocol targeting the 16S rRNA gene is a widely used and cost-effective method for exploring bacterial communities. However, its performance is often limited by primer bias arising from the arbitrary use of universal primers across diverse microbial communities and habitats. We propose KuafuPrimer to design the optimal 16S rRNA gene primers toward minimal bias for targeted bacterial communitie...

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Literature Corpus work
d17b6eda-af38-51bc-95c3-e6670e8857ce
DOI
10.64898/2026.03.29.714677
Open publication

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KuafuPrimer: Machine learning empowers the design of 16S amplicon sequencing primers toward minimal bias for bacterial communitiesDOI 10.64898/2026.03.29.714677
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