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Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16 Feb 2024

Abstract excerpt

BACKGROUND: Accurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) gene sequencing is a widely employed technique for bacterial taxonomic classification, it often results in misclassified or unclassified bacterial taxa. This study sought to refine the full-length 16S rRNA gene sequencing protocol using the...

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PMID: 38365589
DOI: 10.1186/s12866-024-03208-5

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