Back to search

Article

16S rRNA gene and <i>rpoB</i> Nanopore Sequencing for Bacterial Detection and Identification in Clinical Samples

2025-10-01

Abstract excerpt

<h4>ABSTRACT</h4> Broad-range amplification and sequencing of the 16S ribosomal RNA (rRNA) gene directly from clinical samples can improve diagnosis of bacterial infections. Recent advances in the Oxford Nanopore Technologies (ONT) third-generation sequencing platform, delivering sequencing accuracy exceeding 99%, makes it a possible option for this approach. However, even full-length 16S rRNA sequencing may lack...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
dbf6dc4f-ff19-5133-8263-b3a62738644b
DOI
10.1101/2025.09.30.25336965
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
16S rRNA gene and <i>rpoB</i> Nanopore Sequencing for Bacterial Detection and Identification in Clinical SamplesDOI 10.1101/2025.09.30.25336965
Performance and Practicality of 16S Nanopore Sequencing for Routine Bacterial Identification in Clinical SamplesSemantic proximity
Same-Day Simultaneous Diagnosis of Bacterial and Fungal Infections in Clinical Practice by Nanopore Targeted SequencingSemantic proximity
Optimizing and Evaluating Nanopore-Based Targeted and Metagenomic Sequencing Workflows for Rapid Diagnosis of Acute Invasive Infections from Normally Sterile Body FluidsSemantic proximity
Standardization of 16S rRNA gene sequencing using nanopore long read sequencing technology for clinical diagnosis of culture negative infectionsSemantic proximity
The clinical utility of Nanopore 16S rRNA gene sequencing for direct bacterial identification in normally sterile body fluidsSemantic proximity
Implementation of full-length 16S nanopore sequencing for bacterial identification in a clinical diagnostic settingSemantic proximity
Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technologySemantic proximity
The clinical utility of two high-throughput 16S rRNA gene sequencing workflows for taxonomic assignment of unidentifiable bacterial pathogens in MALDI-TOF MSSemantic proximity
Nationwide multicentre study of Nanopore long-read sequencing for 16S rRNA-species identificationSemantic proximity
Rapid nanopore sequencing and predictive susceptibility testing of positive blood cultures from intensive care patients with sepsisSemantic proximity
Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencerSemantic proximity
The application of targeted nanopore sequencing for the identification of pathogens and resistance genes in lower respiratory tract infectionsSemantic proximity
Select a neighboring publication to make it the new centre.