Article

Quantitative microbiome profiling in lumenal and tissue samples with broad coverage and dynamic range via a single-step 16S rRNA gene DNA copy quantification and amplicon barcoding

2020-01-22

Abstract excerpt

<h4>ABSTRACT</h4> Current methods for detecting, accurately quantifying, and profiling complex microbial communities based on the microbial 16S rRNA marker genes are limited by a number of factors, including inconsistent extraction of microbial nucleic acids, amplification interference from contaminants and host DNA, different coverage of PCR primers utilized for quantification and sequencing, and potentially bia...

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Literature Corpus work: ce5e90bf-4f7f-555e-a06f-f39e75512d25
DOI: 10.1101/2020.01.22.914705

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