Article

Adapting CRISPR/Cas9 System for Targeting Mitochondrial Genome

2020-02-12

Abstract excerpt

<h4>ABSTRACT</h4> Gene editing of the mitochondrial genome using CRISPR-Cas9 system is highly challenging mainly due to sub-efficient delivery of guide RNA and Cas9 enzyme complexes into mitochondria. In this study, we were able to perform gene editing in the mitochondrial DNA by appending NADH-ubiquinone oxidoreductase chain 4 (ND4) targeting guide RNA to a RNA transport derived stem loop element (RP-loop) and e...

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Literature Corpus work: 16325ddc-eb42-5d1f-8ad7-5bc2f5adcc05
DOI: 10.1101/2020.02.11.944819

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