Article

Long read mitochondrial genome sequencing using Cas9-guided adaptor ligation

2022-02-24

Abstract excerpt

<h4>ABSTRACT</h4> The mitochondrial genome (mtDNA) is an important source of disease-causing genetic variability, but existing sequencing methods limit understanding, precluding phased measurement of mutations and clear detection of large sporadic deletions. We adapted a method for amplification-free sequence enrichment using Cas9 cleavage to obtain full length nanopore reads of mtDNA. We then utilized the long r...

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Literature Corpus work: 32394342-4639-5307-8466-d371537a11be
DOI: 10.1101/2022.02.23.480720

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