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Article

Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay.

Journal of the American Chemical Society - 12 Sept 2018

Zhang Kaixiang, Deng Ruijie, Teng Xucong, Li Yue, Sun Yupeng, Ren Xiaojun, Li Jinghong

Abstract excerpt

The accumulation of mitochondrial DNA (mtDNA) mutations in cells is strongly related to aging-associated diseases. Imaging of single-nucleotide variation (SNV) in mtDNA is crucial for understanding the heteroplasmy of mtDNAs that harbor pathogenic changes. Herein, we designed a CRISPR/Cas9-mediated proximity ligation assay (CasPLA) for direct visualization of the ND4 and ND5 genes in the mtDNAs of single cells....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 30125486
DOI: 10.1021/jacs.8b05309

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KRAS

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