Article

Alternative strategy to induce CRISPR-mediated genetic changes in hematopoietic cells

2021-10-25

Abstract excerpt

<h4>ABSTRACT</h4> Acute Myeloid Leukaemia is a complex heterogenous disease caused by clonal expansion of undifferentiated myeloid precursors. Recently, several haematological models have been developed with CRISPR/Cas9, using viral vectors, because blood cells are hard to transfect. To avoid virus disadvantages, we have developed a strategy to generate CRISPR constructs, by means of PCR, which any lab equipped w...

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Literature Corpus work: 94b22651-d6ec-58bb-96f2-47562807b228
DOI: 10.1101/2021.10.25.465699

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