Article

Optimizing CRISPR/Cas9 genome editing in primary human hematopoietic cells to advance studies into HIV biology

2026-08-05

Abstract excerpt

Defining how human host factors shape HIV-1 infection in vivo remains essential for the development of genetically engineered cell therapies. Here, we established a non-viral CRISPR/Cas9 ribonucleoprotein-based platform for efficient single and multiplex gene editing in primary human CD34 + hematopoietic stem and progenitor cells (HSPCs). Edited HSPCs retained viability, proliferative capacity, primitive immunop...

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Literature Corpus work: 1fa0235a-99ea-5bed-8307-bcac6a55c546
DOI: 10.64898/2026.08.05.741689

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