Article

Directed-evolution mutations of adenine base editor ABE8e improve its DNA-binding affinity and protein stability

2023-05-09

Abstract excerpt

Adenine base editors (ABEs), consisting of CRISPR-associated (Cas) nickase and deaminase, can convert the A:T base pair to G:C. Previous studies have shown that ABE8e, a directed-evolution variant of ABE7.10 with eight amino-acid mutations in its deaminase TadA-8e, has higher base editing activity than ABE7.10. However, it remains unclear how the directed-evolution mutations of ABE8e increase its editing activity....

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Literature Corpus work: 14cbc6db-e057-5eff-8022-3b4217d3adbd
DOI: 10.1101/2023.05.09.539947

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