Back to search

Article

Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells

2021-09-08

Abstract excerpt

<h4>Background & Aims</h4> 2D monolayers of primary intestinal and colonic epithelial cells represent next-generation in vitro models of the gut. Efficient transgenesis and gene-editing in human intestinal stem cells (hISCs) would significantly improve utility of these models by enabling generation of reporter and loss/gain-of-function hISCs, but no published methods exist for transfecting 2D hISC monolayers. El...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
14241e15-fe58-55c7-a0b0-cfb9dd8a9dd0
DOI
10.1101/2021.09.08.459297
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cellsDOI 10.1101/2021.09.08.459297
Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cellsSemantic proximity
Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPRSemantic proximity
Strategies for genetic manipulation of adult stem cell-derived organoidsSemantic proximity
Ex vivo culture of the intestinal epithelium: strategies and applicationsSemantic proximity
Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloningSemantic proximity
Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem CellsSemantic proximity
Drug Discovery via Human-Derived Stem Cell OrganoidsSemantic proximity
A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in diseaseSemantic proximity
Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive MinigeneSemantic proximity
Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoidsSemantic proximity
A functional genetic toolbox for human tissue-derived organoidsSemantic proximity
A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial OrganoidsSemantic proximity
Select a neighboring publication to make it the new centre.