Article
Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids
27 Jan 2024
Abstract excerpt
Abstract Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC 3D-hIO ) within...
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