Article

ROCK2 Knockout Improves Proliferation Rate in a Cellular Model of Down Syndrome

2022-10-21

Abstract excerpt

<h4>ABSTRACT</h4> In a cellular model of Down Syndrome, hTERT immortalized RPE-1 (human retinal pigment epithelial-1) cells carrying an extra copy of chromosome 21 exhibit reduced fitness, in part, as an increase in doubling time (or a reduction in cell proliferation rate) in culture. ROCK2 (Rho associated coiled-coil containing kinase 2) was identified in a whole genome CRISPR knockout (KO) screen designed to id...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 121d2a33-0733-5c98-a385-ba0f8d65b4cd
DOI: 10.1101/2022.10.20.513071

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

ROCK2 Knockout Improves Proliferation Rate in a Cellular Model of Down SyndromeDOI 10.1101/2022.10.20.513071Read

Anti-inflammatory and pro-proliferative effects of fasudil in human trisomy 21 neural progenitor cellsSemantic proximity Read

Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the Diversity of p53-Dependent Responses to Cell-Cycle Defects.Semantic proximity Read

Chromosome silencing <i>in vitro</i> reveals trisomy 21 causes cell-autonomous deficits in angiogenesis and early dysregulation in Notch signalingSemantic proximity Read

An efficient method for immortalizing mouse embryonic fibroblastsSemantic proximity Read

Engineered cartilage from human chondrocytes with homozygous knockout of cell cycle inhibitor p21Semantic proximity Read

Engineered Cartilage from Human Chondrocytes with Homozygous Knockout of Cell Cycle Inhibitor p21Semantic proximity Read

Congenital heart defects in Down syndrome are caused by increased dosage of DYRK1ASemantic proximity Read

Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndromeSemantic proximity Read

Rack1 is essential for corticogenesis by preventing p21-dependent senescence in neural stem cells.Semantic proximity Read

Restoration of keratinocytic phenotypes in autonomous trisomy-rescued cells.Semantic proximity Read

WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility.Semantic proximity Read

Trisomy 21 increases microtubules and disrupts centriolar satellite localizationSemantic proximity Read