Article

Ubi-tagging: A ubiquitin-based modular platform for site-directed antibody conjugation

2022-07-07

Abstract excerpt

<title>Abstract</title> <p>Antibody conjugates form a foundation for many research, diagnostic, and therapeutic applications. Despite the robustness and efficiency of existing antibody conjugation techniques, the challenge of obtaining homogeneous products remains. Here, we developed a versatile modular method for site-directed antibody conjugation using the small protein ubiquitin. We show that ubiquitin, when f...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 111170a5-0ebb-59ec-8502-adb5efc72e02
DOI: 10.21203/rs.3.rs-1835302/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Ubi-tagging: A ubiquitin-based modular platform for site-directed antibody conjugationDOI 10.21203/rs.3.rs-1835302/v1Read

High-Throughput Platform to Identify Antibody Conjugation Sites from Antibody-Drug Conjugate Libraries.Semantic proximity Read

Antibody and antibody fragments Site Specific Conjugation using New Q-tag Substrate of Bacterial Transglutaminase.Semantic proximity Read

Affinity‐Directed Site‐Specific Protein Labeling and Its Application to Antibody‐Drug ConjugatesSemantic proximity Read

Antibody Conjugates with Unnatural Amino AcidsSemantic proximity Read

Functional ubiquitin conjugates with lysine-epsilon-amino-specific linkage by thioether ligation of cysteinyl-ubiquitin peptide building blocks.Semantic proximity Read

Chemical synthesis of ubiquitin, ubiquitin-based probes, and diubiquitin.Semantic proximity Read

Advances in nanobody multimerization and multispecificity: from in vivo assembly to in vitro productionSemantic proximity Read

Engineering the orthogonal ubiquitin E1-E2 pairs for identification of K27 chain linkage substrates.Semantic proximity Read

Site-specific photocoupling of pBpa mutated scFv antibodies for use in affinity proteomics.Semantic proximity Read

Programable Albumin-Hitchhiking Nanobodies Enhance the Delivery of STING Agonists to Potentiate Cancer ImmunotherapySemantic proximity Read

A Plug-and-Play Approach for the De Novo Generation of Dually Functionalised BispecificsSemantic proximity Read

Modular construction of bispecific antibodies through bioconjugation for T cell-based immunotherapySemantic proximity Read