Article
Site-specific photocoupling of pBpa mutated scFv antibodies for use in affinity proteomics.
Biochimica et biophysica acta. Proteins and proteomics - 1 Aug 2017
Brofelth Mattias, Städe Lars Wagner, Ekstrand Anna Isinger, Edfeldt Linn Petersson, Kovačič Rebeka, Nielsen Thorbjørn Terndrup, Larsen Kim Lambertsen, Duroux Laurent, Wingren Christer
Abstract excerpt
Recombinant antibody libraries can provide a source of renewable and high-performing binders tailored for use in affinity proteomics. In this context, the process of generating site-specific 1:1 tagging/functionalization and/or orientated surface immobilization of antibodies has, however, proved to be challenging. Hence, novel ways of generating such engineered antibodies for use in affinity proteomics could have...
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