Article

Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase

2021-09-20

Abstract excerpt

Efficient, targeted integration of large DNA constructs represent a significant hurdle in genetic engineering for the development of mouse models of human disease and synthetic biology research. To address this, we developed a system for efficient and precise, targeted single-copy integration of large transgenes directly into the zygote using multiple mouse genetic backgrounds. Conventional approaches, such as ran...

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Literature Corpus work: 6f6e2bd4-2933-5ffb-b1c5-c87840c5ecc7
DOI: 10.1101/2021.09.20.461117

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