Article

Cell-free transcriptional regulation via nucleic-acid-based transcription factors

2019-05-20

Abstract excerpt

Cells execute complex transcriptional programs by deploying distinct protein regulatory assemblies that interact with cis-regulatory elements throughout the genome. Using concepts from DNA nanotechnology, we synthetically recapitulated this feature in cell-free gene networks actuated by T7 RNA polymerase (RNAP). Our approach involves engineering nucleic-acid hybridization interactions between a T7 RNAP site-specif...

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Literature Corpus work: 0bfb255e-f86f-547e-b861-d8e42a9f58c3
DOI: 10.1101/644021

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