Back to search

Article

Single nuclei transcriptomics of muscle reveals intra-muscular cell dynamics linked to dystrophin loss and rescue

2022-05-31

Abstract excerpt

In Duchenne muscular dystrophy, dystrophin loss leads to chronic muscle damage, dysregulation of repair, fibro-fatty replacement, and weakness. We develop methodology to efficiently isolate individual nuclei from frozen skeletal muscle, allowing single nuclei sequencing of irreplaceable archival samples from small samples. We apply this method to identify cell and gene expression dynamics within human DMD and mdx...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
0613553b-d0ed-5526-be55-444608609cdb
DOI
10.1101/2022.05.31.494197
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Single nuclei transcriptomics of muscle reveals intra-muscular cell dynamics linked to dystrophin loss and rescueDOI 10.1101/2022.05.31.494197
Single nuclei transcriptomics of muscle reveals intra-muscular cell dynamics linked to dystrophin loss and rescueSemantic proximity
Single nuclei/cell transcriptomics reveal DMD driven cell dynamics and mechanisms of fibroblast inflammatory tissue priming in human dystrophic muscleSemantic proximity
Degenerative and regenerative pathways underlying Duchenne muscular dystrophy revealed by single-nucleus RNA sequencing.Semantic proximity
Decoding Duchenne muscular dystrophy transcriptome to single nuclei level reveals clinical-genetic correlationsSemantic proximity
Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cellsSemantic proximity
A high-resolution single-nucleus RNA sequencing reveals conserved macrophage activation trajectories across muscle regeneration and dystrophy.Semantic proximity
Unraveling the spatial landscape of Dystrophinopathies: a transcriptomic approach to Becker and Duchenne muscular dystrophiesSemantic proximity
Transcriptomic analysis of paired healthy human skeletal muscles to identify modulators of disease severity in DMDSemantic proximity
Muscle stem cells in Duchenne muscular dystrophy exhibit molecular impairments and altered cell fate trajectories impacting regenerative capacitySemantic proximity
Muscle stem cells in Duchenne muscular dystrophy exhibit molecular impairments and altered cell fate trajectories impacting regenerative capacitySemantic proximity
Single cell RNA sequencing of human FAPs reveals different functional stages in Duchenne muscular dystrophySemantic proximity
Single-Nucleus RNA-Sequencing Profiling of Mouse Lung. Reduced Dissociation Bias and Improved Rare Cell-Type Detection Compared with Single-Cell RNA SequencingSemantic proximity
Select a neighboring publication to make it the new centre.