3'-Azido-3'-deoxythymidine-resistant mutants of DNA polymerase beta identified by in vivo selection.

doi:10.1074/jbc.274.6.3851

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Article

3'-Azido-3'-deoxythymidine-resistant mutants of DNA polymerase beta identified by in vivo selection.

The Journal of biological chemistry - 5 Feb 1999

Kosa J L, Sweasy J B

Abstract excerpt

We developed an in vivo selection to identify 3'-azido-3'-deoxythymidine (AZT)-resistant mutants of rat DNA polymerase beta (pol beta). The selection utilizes pol beta's ability to substitute for Escherichia coli DNA polymerase I (pol I) in the SC18-12 strain, which lacks active pol I. pol beta a...

Topics

Animals
Antiviral Agents
Base Sequence
Catalysis
Cell Line
DNA Polymerase beta
DNA Primers
Drug Resistance, Microbial
Genetic Complementation Test
Mutation
Phenotype
Protein Conformation
Rats
Substrate Specificity
Zidovudine

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