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Article

3'-Azido-3'-deoxythymidine-resistant mutants of DNA polymerase beta identified by in vivo selection.

The Journal of biological chemistry - 5 Feb 1999

Kosa J L, Sweasy J B

Abstract excerpt

We developed an in vivo selection to identify 3'-azido-3'-deoxythymidine (AZT)-resistant mutants of rat DNA polymerase beta (pol beta). The selection utilizes pol beta's ability to substitute for Escherichia coli DNA polymerase I (pol I) in the SC18-12 strain, which lacks active pol I. pol beta allows SC18-12 cells to grow, but they depend on pol beta activity, so inhibition of pol beta by AZT kills them. We...

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PMID: 9920940
DOI: 10.1074/jbc.274.6.3851

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