Article
PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1.
Nucleic acids research - 1 Jan 2004
Sismour A Michael, Lutz Stefan, Park Jeong-Ho, Lutz Michael J, Boyer Paul L, Hughes Stephen H, Benner Steven A
Abstract excerpt
As the next step towards generating a synthetic biology from artificial genetic information systems, we have examined variants of HIV reverse transcriptase (RT) for their ability to synthesize duplex DNA incorporating the non-standard base pair between 2,4-diaminopyrimidine (pyDAD), a pyrimidine presenting a hydrogen bond 'donor-acceptor-donor' pattern to the complementary base, and xanthine (puADA), a purine...
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