Selection of new biologically active molecules from random nucleotide sequences.

doi:10.1016/0378-1119(93)90249-3

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Article

Selection of new biologically active molecules from random nucleotide sequences.

Gene - 27 Dec 1993

Dube D K, Black M E, Munir K M, Loeb L A

Abstract excerpt

Genetic diversity can be achieved in vitro by inserting random nucleotide (nt) sequences into cloned genes. In the case of enzymes, subsequent genetic complementation can be used to select for new mutants that exhibit different substrate specificities, altered catalytic activities, or altered tem...

Topics

Base Sequence
Binding Sites
Consensus Sequence
Genetic Engineering
Genetic Variation
Mutation
Random Allocation
Recombination, Genetic
Regulatory Sequences, Nucleic Acid
Simplexvirus
Thymidine Kinase
beta-Lactamases

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