Article
Selection of new biologically active molecules from random nucleotide sequences.
Gene - 27 Dec 1993
Dube D K, Black M E, Munir K M, Loeb L A
Abstract excerpt
Genetic diversity can be achieved in vitro by inserting random nucleotide (nt) sequences into cloned genes. In the case of enzymes, subsequent genetic complementation can be used to select for new mutants that exhibit different substrate specificities, altered catalytic activities, or altered temperature sensitivities. Using this technique, one can also analyze the contribution of different amino acid residues to...
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