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Article

Enzymatic properties of rat DNA polymerase beta mutants obtained by randomized mutagenesis.

Nucleic acids research - 1 Jun 2001

Skandalis A, Loeb L A

Abstract excerpt

We have used random sequence mutagenesis to generate mutants of DNA polymerase beta in an effort to identify amino acid residues important for function, catalytic efficiency and fidelity of replication. A library containing 100 000 mutants at residues 274-278 in the N-helix of the thumb subdomain of the polymerase was constructed and screened for polymerase activity by genetic complementation. The genetic screen...

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Identifiers

PMID: 11376161
DOI: 10.1093/nar/29.11.2418
PMCID: PMC55708

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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