Conversion of thymidylate synthase into an HIV protease substrate.

Abstract excerpt

Thymidylate synthase (TS) is an essential enzyme of DNA metabolism. We have carried out an extensive insertional mutagenesis of the Escherichia coli TS gene (thyA) using three different methods. Insertion of exogenous sequences at unique restriction sites or at random positions produced defective...

Topics

• Amino Acid Sequence
• Binding Sites
• Escherichia coli
• Genes, Bacterial
• HIV Protease
• Humans
• Molecular Sequence Data
• Mutagenesis, Insertional
• Phenotype
• Substrate Specificity
• Thymidylate Synthase