Article
A mutant of BamHI restriction endonuclease which requires N6-methyladenine for cleavage.
Journal of molecular biology - 29 Jan 1999
Whitaker R D, Dorner L F, Schildkraut I
Abstract excerpt
Amino acid residues Asn116 and Ser118 of the restriction endonuclease BamHI make several sequence-specific and water-bridged contacts to the DNA bases. An in vivo selection was used to isolate BamHI variants at position 116, 118 and 122 which maintained sequence specificity to GGATCC sites. Here,...
Topics
- Adenine
- Base Sequence
- Binding Sites
- Binding, Competitive
- DNA, Bacterial
- Deoxyribonuclease BamHI
- Escherichia coli
- Genetic Variation
- Hydrogen Bonding
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Conformation
- Protein Engineering