Article
EcoKI with an amino acid substitution in any one of seven DEAD-box motifs has impaired ATPase and endonuclease activities.
Nucleic acids research - 1 Nov 1998
Davies G P, Powell L M, Webb J L, Cooper L P, Murray N E
Abstract excerpt
For type I restriction systems, recently determined nucleotide sequences predict conserved amino acids in the subunit that is essential for restriction but not modification (HsdR). The conserved sequences emphasize motifs characteristic of the DEAD-box family of proteins which comprises putative...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Sequence
- Amino Acid Substitution
- Base Sequence
- DNA Primers
- DNA Restriction Enzymes
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Protein Conformation
- Restriction Mapping