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Article

EcoKI with an amino acid substitution in any one of seven DEAD-box motifs has impaired ATPase and endonuclease activities.

Nucleic acids research - 1 Nov 1998

Davies G P, Powell L M, Webb J L, Cooper L P, Murray N E

Abstract excerpt

For type I restriction systems, recently determined nucleotide sequences predict conserved amino acids in the subunit that is essential for restriction but not modification (HsdR). The conserved sequences emphasize motifs characteristic of the DEAD-box family of proteins which comprises putative helicases, and they identify a new candidate for motif IV. We provide evidence based on an analysis of Eco KI which...

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Identifiers

PMID: 9776741
DOI: 10.1093/nar/26.21.4828
PMCID: PMC147943

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