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Article

Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI.

Scientific reports - 7 Mar 2014

Horton John R, Nugent Rebecca L, Li Andrew, Mabuchi Megumu Yamada, Fomenkov Alexey, Cohen-Karni Devora, Griggs Rose M, Zhang Xing, Wilson Geoffrey G, Zheng Yu, Xu Shuang-yong, Cheng Xiaodong

Abstract excerpt

The modification-dependent restriction endonuclease AspBHI recognizes 5-methylcytosine (5mC) in the double-strand DNA sequence context of (C/T)(C/G)(5mC)N(C/G) (N = any nucleotide) and cleaves the two strands a fixed distance (N12/N16) 3' to the modified cytosine. We determined the crystal structure of the homo-tetrameric AspBHI. Each subunit of the protein comprises two domains: an N-terminal DNA-recognition...

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PMID: 24604015
DOI: 10.1038/srep04246
PMCID: PMC3946040

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