Article
The core element of the EcoRII methylase as defined by protease digestion and deletion analysis.
Nucleic acids research - 11 Oct 1991
Friedman S, Som S, Yang L F
Abstract excerpt
Binding of the EcoRII DNA methyltransferase to azacytosine-containing DNA protects the enzyme from digestion by proteases. The limit digest yields a product having a Mr on SDS-PAGE 20% less than the intact protein. The N terminus of the tryptic digestion product was sequenced and found to be missing the N terminal 82 amino acids. Under the conditions used unbound enzyme was digested to small peptides. Protection...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Deletion
- Cytosine
- DNA Mutational Analysis
- DNA, Bacterial
- DNA-Cytosine Methylases
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Escherichia coli
- Kinetics