Article
An alternative approach for screening active bam HI variants: overexpression in T-7 RNA polymerase based system.
Indian journal of biochemistry & biophysics - 1 Oct 2001
Acharya A S, Roy K B
Abstract excerpt
The type II restriction endonuclease, Bam HI, has been overexpressed in E. coli by cloning the Bam HI gene in frame with an E. coli Ribosome Binding Site (RBS) under the T7 promoter of an E. coli expression vector pRSET A. The expression level of Bam HI endonuclease using this construct was found to be higher than that reported of the overexpressing clone pAEK14. Our overexpressing clone, pAABRw in BL21 cells in...
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