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Article

Changing the target base specificity of the EcoRV DNA methyltransferase by rational de novo protein-design.

Nucleic acids research - 1 Aug 2001

Roth M, Jeltsch A

Abstract excerpt

The EcoRV DNA-(adenine-N(6))-methyltransferase (M.EcoRV) specifically modifies the first adenine residue within GATATC sequences. During catalysis, the enzyme flips its target base out of the DNA helix and binds it into a target base binding pocket which is formed in part by Lys16 and Tyr196. A cytosine residue is accepted by wild-type M.EcoRV as a substrate at a 31-fold reduced efficiency with respect to the...

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Identifiers

PMID: 11470870
DOI: 10.1093/nar/29.15.3137
PMCID: PMC55820

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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