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Article

A monomeric mutant of restriction endonuclease EcoRI nicks DNA without sequence specificity.

Biological chemistry - 1 Oct 2004

Fritsche Petra, Alves Jürgen

Abstract excerpt

We have mutated the monomer-monomer interface of the restriction endonuclease EcoRI in order to destabilize the homodimer and to stabilize heterodimers. Mutations of Leu158 to charged amino acid residues result in strong destabilization of the dimer. The largest effect was detected for the L158D mutant which is monomeric even at higher concentrations. It unspecifically degrades DNA by cleaving both single strands...

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Source

Identifiers

PMID: 15551873
DOI: 10.1515/BC.2004.127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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