Article
Cyclin B-cdk1 kinase stimulates ORC- and Cdc6-independent steps of semiconservative plasmid replication in yeast nuclear extracts.
Molecular and cellular biology - 1 Feb 1999
Duncker B P, Pasero P, Braguglia D, Heun P, Weinreich M, Gasser S M
Abstract excerpt
Nuclear extracts from Saccharomyces cerevisiae cells synchronized in S phase support the semiconservative replication of supercoiled plasmids in vitro. We examined the dependence of this reaction on the prereplicative complex that assembles at yeast origins and on S-phase kinases that trigger ini...
Topics
- Animals
- CDC2 Protein Kinase
- Cell Cycle Proteins
- Cell Nucleus
- Cyclin B
- DNA Replication
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- G1 Phase
- In Vitro Techniques
- Mutation
- Origin Recognition Complex
- Plasmids
- Recombinant Proteins